Enhanced Lipid Production under High Light Intensity High to Low Nitrogen Concentration

Dunaliella salina (D. salina) is a potential source of lipids and subsequent biodiesel production. However lipid production from dependent on biomass production rate and lipid content. To get more lipid, production efficiency need to be improved. The aim was to application of two-stage culture strategy for improved microalgal growth and lipid Production. In this study the culture was grown under four different nitrogen concentrations (2 mM) to low nitrogen (2 mM) concentration under high (800 to get the high lipid yield. The result showed that maximum lipid contents (46%) and lipid per cell (37 culture grown under low nitrogen concentration whereas high nitrogen culture showed maximum lipid concentration 0.73 g L productivity 56.32 mg Lday when grown under high light intensity. The culture grown increased the overall lipid productivity due to increase the biomass dry weight (2.13 g L However the culture shifted from high nitrogen to low nitrogen concent of lipids (56.47%), concentration of lipid (1.169 g L shifting of D. salina culture from high to low nit overall lipid production in D. salina. This strategy can be used for outdoor cultivation for efficient algal biofuels production.